This study aimed at investigating the effect of organization culture, team effectiveness, team characteristics, supervision, and interpersonal relation on team effectiveness as well as their impacts on organization culture at State University of Medan, Indonesia. Population of this study were all academic units (n=311) at the university particularly ranging from university’s administrators who serve as structural officials and lecturers who serve as administrative officials. In the interest of subject of the study, random sampling technique on the basis of Cochran formula was employed, and thus resulted in 155 people (49.84%). Data were obtained from close-ended questionnaire, and were analyzed under the auspices of Structural Equation Modeling (SEM) analysis with PLS smart software program. The result turned out that four out of seven hypotheses were accepted, whereas the rest of it was rejected. More specifically, the four-accepted hypotheses were found directly in the effect of organization culture on team effectiveness (λ=.685), team characteristics on organization culture (λ=.233), supervision on organization culture (λ=.301), and interpersonal relation on organization culture (λ=.373). However, the three-rejected hypotheses were found directly in the effect of team characteristics on team effectiveness (λ=.063), supervision on team effectiveness (λ=.087), and interpersonal relation on team effectiveness (λ=.114). Notwithstanding such effects, this indicated other indirect significant effects through organization culture, particularly in the indirect effect of team characteristics on team effectiveness (λ=.223), supervision on team effectiveness (λ=.293), and interpersonal relation on team effectiveness (λ=.370). All in all, it was reasonable to conclude that the development of team effectiveness at State University of Medan was achievable in the revitalization of organization culture. In the interest of powerful organization culture, it was necessary to pay attention painstakingly to team characteristics, conduct supervision, and create conducive interpersonal relation.